The San Francisco 49ers held the 10th overall selection in the 2009 NFL draft. With the 10th pick, the 49ers selected WR Michael Crabtree from Texas Tech.

In the 2008 season, the 49ers finished 7-9 in the regular season, finishing in second place in the NFC West. This is their first draft under new head coach Mike Singletary, who replaced Mike Nolan on an interim basis during the 2008 season, then was awarded the full-time head coaching position.

Priot to 2009, the 49ers had used three of their past four first-round selections on defensive players, including 2008, when they selected North Carolina defensive end Kentwan Balmer with the 29th overall selection.
- The 49ers traded the 11th pick in the 2nd round (43rd overall) and the 11th pick in the 4th round (111th overall) to the Carolina Panthers in exchange for a first round pick in 2010 on April 25, 2009.